Lyophilized (freeze-dried) research peptides must be reconstituted with an appropriate solvent before use in laboratory experiments. This guide covers standard practices used in research settings.
What You Need
- Lyophilized peptide vial (from a supplier with COA)
- Bacteriostatic water (0.9% benzyl alcohol) — most common for multi-dose vials
- Sterile insulin syringe (U-100, 100 units = 1 mL)
- Alcohol swabs for vial tops
- Clean laboratory workspace
Basic Reconstitution Steps
- Allow the lyophilized vial to reach room temperature (15–30 minutes).
- Swab the rubber stopper with alcohol and let dry.
- Draw the desired volume of bacteriostatic water into the syringe.
- Inject water slowly down the inside wall of the vial — do not spray directly onto the powder.
- Gently swirl (do not shake vigorously) until fully dissolved.
- Label with date, concentration, and compound name. Store at 2–8 °C.
Calculating Concentration
Concentration (mg/mL) = total peptide mass (mg) ÷ water volume added (mL). Example: 10 mg peptide + 2 mL water = 5 mg/mL. Our compound guides include pre-calculated syringe unit charts for common reconstitutions.
Storage After Reconstitution
Reconstituted peptides should be refrigerated at 2–8 °C and used within 3–4 weeks. Avoid repeated freeze-thaw cycles. See our full storage guide.
Research use only. Products are not approved for human or veterinary consumption. For customers aged 21+ aged 21+.