TB-500 (Thymosin Beta-4 Fragment)
A fragment of a natural healing protein that helps cells move and repair tissue in lab studies.
What Researchers Study
TB-500 is a small active piece of a larger protein called thymosin beta-4. Researchers study it because it helps cells change their internal "skeleton" (actin), which lets them move around better. This makes it interesting for wound healing, tissue repair, and situations where cells need to travel to an injury site.
Primary Research Applications
- Scratch tests to see how fast cells can close a wound gap
- Watching how cells reorganize their internal structure
- Eye and skin cell movement experiments
- Heart and blood vessel repair models
Benefits Researchers Are Exploring (in simple terms)
- Helps cells (especially skin and blood vessel cells) move and crawl to where they are needed in healing models.
- Supports the formation of new blood vessels that can supply oxygen and nutrients to damaged tissue.
- Often linked with faster wound closure and better organization of new tissue in lab and tissue studies.
- May reduce some signs of inflammation in injured tissue models.
- Very commonly paired with BPC-157 because the two appear to support different but complementary parts of the repair process.
Mechanisms Under Investigation
Actin and Cell Skeleton
It binds to a building block of the cell skeleton (G-actin) and helps turn it into longer filaments. This gives cells the "legs" they need to move.
Better Cell Travel
In scratch assays (a common lab wound model), cells treated with TB-500 close the gap faster because they can migrate more effectively.
Supports Blood Vessel Growth
It is studied for helping endothelial cells form tube-like structures, which is an early step in growing new blood vessels.
Reconstitution Reference (how to mix it for lab use)
| Common vial sizes | 5 mg and 10 mg |
| Reconstitution | Add 2 mL bacteriostatic water to a 5 mg vial = 2.5 mg per mL |
| Storage | Freeze the powder. After mixing, keep refrigerated. |
| Typical lab concentrations | 0.1 to 5 micrograms per milliliter in cell culture work |
Dosing Schedule Options from Research
Example Options Used in Studies
- Standard research option: Draw **80-200 units** (2-5 mg) on your 100-unit syringe twice per week (e.g. Mon/Thu) for 4-6 weeks in soft tissue repair studies. Use 5 mg vial + 2 mL BAC = 2.5 mg/mL concentration.
- Daily lower dose: Draw **10-20 units** (250-500 mcg) every day for 2-4 weeks (common in shorter cell culture or tissue model work) at 2.5 mg/mL.
- Combined with BPC-157: Draw **80-200 units** TB-500 (2-5 mg) twice weekly + 10-14 units BPC-157 (250-350 mcg) daily for 4-8 weeks.
- Loading approach: 4-8 mg per week (160-320 units at 2.5 mg/mL) split into 2 doses for first 2 weeks, then drop to maintenance.
100-Unit Insulin Syringe Conversion Chart
Reconstitution used for these calculations: 5 mg vial + 2 mL bacteriostatic water = 2.5 mg/mL concentration (standard for easy 100-unit syringe dosing).
Important: We have already done all the math for you. U-100 insulin syringes are marked in "units" (100 units = 1 mL). Just draw the exact number of units listed below for each dose. No calculations required on your end.
| Dose (mg) | Units on 100-unit syringe (pre-calculated) |
|---|---|
| 2 mg (standard weekly split) | 80 units (0.80 mL) per dose |
| 2.5 mg (common) | 100 units (1.00 mL) per dose |
| 5 mg (higher weekly) | 200 units (2.00 mL) per dose |
| 250-500 mcg daily | 10-20 units (0.10-0.20 mL) |
These unit amounts assume the exact concentration listed above. Always double-check your own reconstitution volume against the chart.
Safe Research Cycle Guidelines
Example Cycle References from Research Literature
- Typical research cycle: 4-6 weeks on at 2-5 mg twice weekly, followed by at least 4 weeks off.
- Combined protocol: 4-8 weeks with BPC-157, minimum 4 weeks recovery between cycles.
- Key: Never exceed recommended research ranges. Follow approved institutional protocols and primary literature.
Research Notes
TB-500 is mainly studied for its ability to help cells move and support repair. Because it is often used together with BPC-157, many published references describe combination protocols rather than TB-500 by itself.
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